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. 2021 Nov 24;221(2):e202107070. doi: 10.1083/jcb.202107070

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© 2021 Crosby et al.

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Figure S4. — cyt-ATL2 has single turnover kinetics of dimerization and crossover formation similar to those of cyt-ATL1 and cyt-DATL. (A) Schematic of FRET as cyt-ATL monomers labeled with a FRET donor and acceptor on the G domain (i) undergo G domain dimerization (G-G FRET; ii). (B) Comparison of G-G acceptor FRET for cyt-ATL2, cyt-ATL1, and cyt-DATL after GTP addition under stopped flow. norm., normalized. (C) Schematic of Cy3 fluorescence enhancement as Cy3-labeled cyt-ATL2, cyt-ATL1, and cyt-DATL (i) undergo dimer formation and crossover (C/O PIFE; ii). (D) Comparison of crossover PIFE for cyt-ATL2, cyt-ATL1, and cyt-DATL after GTP addition under stopped flow. All reactions contained 15 µM cyt-ATL and 7.5 µM GTP (final) and were at 25°C. All traces are the average of three to five individual traces and normalized (initial value = 0; maximum value = 1).