Figure 1.
PPEP-1 bioluminescent sensor designs. (A) Sensor designs based on NanoBiT. LgBiT and SmBit fragments were separated via linkers comprising PPEP-1 cleavage motifs. KD values denote the SmBiT peptide sequence used in each design. Scissile bonds are denoted by triangles. (B) Sensor design based on a caged version of Nluc. A caging peptide was fused to the C-terminus of Nluc via a PPEP-1 cleavage sequence. PPEP-1 releases the cage, turning on Nluc activity. Light is thus produced in the presence of furimazine.