FIG. 2.

(A) Cloning of BUR2. Plasmids containing subclones derived from the original bur2-complementing plasmid were tested for complementation of bur2 phenotypes. The parental plasmid (pGP92) is shown at the top; ORFs are designated by arrows, and nucleotide positions on chromosome XII are shown just below the ends of the fragment. In the rightmost column, + indicates complementation of all bur2 phenotypes and − indicates inability to complement bur2. (B) bur2 null phenotype. Ten tetrads were dissected from a diploid strain that is heterozygous for the bur2 deletion allele bur2Δ2::TRP1. The four spores (a to d) from each tetrad produced two healthy colonies and two slowly growing colonies. Each of the slowly growing colonies was Trp⁺, indicating that the growth defect was caused by bur2Δ.