FIG. 4.

Two-hybrid analysis. Reporter strain PJ69-4A was transformed with plasmids that express GAL4AD or GAL4BD fusions to BUR1 or BUR2 as indicated at the top. A − indicates the presence of GAL4AD or GAL4BD vector controls. Transformants were replica plated to complete medium and medium lacking histidine (−His). Growth on −His plates indicates a positive interaction by this assay. The Gal4BD-p53 and Gal4AD-T antigen (TAg) fusions on the left served as a positive control for interacting hybrid proteins.