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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: FASEB J. 2021 Nov;35(11):e21962. doi: 10.1096/fj.202100936R

Figure 4: iRhom1-dependent brain shedding substrates are substrates for ADAM17.

Figure 4:

Adam17+/+ (black) and Adam17−/− (red) MEFs overexpressing substrate candidates carrying an alkaline phosphatase (AP)-tag were treated with or without PMA for 45 min (N=3). The AP activity in the conditioned media was normalized to the expression level as well as to the untreated sample. P-values indicated in brackets for all significantly upregulated shed ectodomains upon PMA stimulation: TGFα (1.13E-03), EPHB6 (1.49E-03), MEGF10 (1.42E-04), MRC2 (3.75E-04), SEMA5B (2.9E-04) (unpaired t-test). (** p-value < 0.01, *** p-value < 0.001)