Figure 5: T cell-intrinsic suppression of Mtb-specific TCR transgenic T cells.

A-F: Mice infected with 50–100 CFU Mtb, received 10⁴ naïve Tg.WT and Tg.KO cells at d11 p.i. Lungs taken at specified timepoints. (n=4) A) Number of IV⁻ Tg.WT and Tg.KO cells detected. B) Proportion of parenchymal Tg.WT and Tg.KO cells that are Annexin V⁺, SYTOX⁻ (left), or Ki67⁺ (right). C) Proportion of parenchymal Tg.WT and Tg.KO cells that are KLRG1⁺ D) Proportion of parenchymal KLRG1⁺ Tg.WT and Tg.KO cells that are Ki67⁺ E) T-bet MFI of parenchymal Tg.WT and Tg.KO cells F) Proportion of parenchymal Tg.WT and Tg.KO cells that are IFNγ⁺ (G-I) Mice infected with ULD Mtb, received 10⁶ each Tg.WT and Tg.KO Th1-polarized CD4 T cells at d34 p.i. Lungs harvested 20 hours after transfer. n=6 G) Number of cells detected per granuloma section H) Representative section (left) demonstrating presence of Tg.WT (green) and Tg.KO (blue) cells within the granuloma. Zoom-in panel (right) showing localization of both cell types proximal to Mtb-infected cells, as well as demonstrating IFNγ staining. I) Histocytometry quantification of pS6, IFNγ and Ki67 staining in Tg.WT vs Tg.KO transferred cells within the granuloma. J-L: Mice infected with ULD Mtb and starting d14 p.i., received two adoptive transfers of either Tg.WT or Tg.KO Th1-polarized cells (and no-transfer controls), spaced one week apart. Lungs harvested 3–4 days after last transfer. (n=17–20 per group) J) Number of Tg.WT or Tg.KO cells per million lymphocytes. K) Proportion of parenchymal Tg.WT and Tg.KO cells that are KLRG1⁺ L) Number of IFNγ⁺ Tg.WT or Tg.KO cells per million lymphocytes. Single-group comparisons by paired t test (A-G, I) and unpaired T test (J-L). Data are representative of two (H-L) or one (A-G) independent experiments. Points are individual mice, lines (when present) connect Tg.WT and Tg.KO cells within the same host animals. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. See also Figure S5.