KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD11c BV480 | BD; clone HL3 | Cat# 565627, RRID: AB_2739309 |
| Anti-mouse CD11b BV510 | BioLegend; clone M1/70 | Cat# 101263, RRID: AB_2629529 |
| Anti-mouse CD11b BV650 | BioLegend; clone M1/70 | Cat# 101239, RRID: AB_11125575 |
| Anti-mouse CD11b PerCP-Cy5.5 | BioLegend; clone M1/70 | Cat# 101228; RRID: AB_893232 |
| Anti-mouse CD45.1 BV421 | BioLegend; clone A20 | Cat# 110732, RRID:AB_2562563 |
| Anti-mouse CD45.1 APC | BioLegend; clone A20 | Cat# 110713, RRID:AB_313502 |
| Anti-mouse CD45.1 APCeF780 | Thermo Fisher; clone A20 | Cat# 47-0453-82, RRID:AB_1582228 |
| Anti-mouse Ly6G BV650 | BioLegend; clone IA8 | Cat# 127641, RRID:AB_2565881 |
| Anti-mouse Ly6G PerCP-Cy5.5 | BioLegend; clone IA8 | Cat# 127616, RRID:AB_1877271 |
| Anti-mouse CD45.2 AF700 | BioLegend; clone 104 | Cat# 109822, RRID:AB_493731 |
| Anti-mouse CD45.2 APC | BioLegend; clone 104 | Cat# 109813, RRID:AB_389210 |
| Anti-mouse MHCII AF700 | Thermo Fisher; clone M5/114.15.2 | Cat# 56-5321-82, RRID:AB_49400 |
| Anti-mouse CD3 CF633 [conjugated in house] | BioLegend; clone 17A2 | 100202, RRID:AB_312659 |
| Anti-mouse CD3 BV480 | BD; clone 17A2 | Cat# 565642, RRID:AB_2739318 |
| Anti-mouse CD3 BV786 | BD; clone 17A2 | Cat# 564010, RRID:AB_2738540 |
| Anti-mouse CD8a, BV650 | BD; clone 53-6.7 | Cat# 563234, RRID:AB_2738084 |
| Anti-mouse CD8a, PerCP-Cy5.5 | BioLegend; clone 53-6.7 | Cat# 100734, RRID:AB_2075238 |
| Anti-mouse IFNγ PE | BioLegend; clone XMG1.2 | Cat# 505807, RRID:AB_315401 |
| Anti-mouse IFNγ PE-Cy7 | Thermo Fisher; clone XMG1.2 | Cat# 505826, RRID:AB_2295770 |
| Anti-mouse IFNγ AF488 | Thermo Fisher; clone XMG1.2 | Cat# 505813, RRID:AB_493312 |
| Anti-mouse pS6 AF488 | Cell Signaling; clone 2F9 | Cat# 4854, RRID:AB_390782 |
| Anti-mouse CD4 APC | BioLegend; clone RM4-4 | Cat# 116013, RRID:AB_2563024 |
| Anti-mouse CD4 PE | BioLegend; clone RM4-4 | Cat# 116005, RRID:AB_313690 |
| Anti-mouse CD4 CF660 [conjugated in house] | BioLegend; clone RM4-5 | Cat# 100506, RRID:AB_312709 |
| Anti-mouse CD4 e506 | Thermo Fisher; clone RM4-5 | Cat# 69-0042-82, RRID:AB_2637459 |
| Anti-mouse CD4 CF594 [conjugated in house] | BioLegend; clone RM4-5 | Cat# 100506, RRID:AB_312709 |
| Anti-mouse CD4 BV510 | BioLegend; clone RM4-5 | Cat# ; RRID: AB_2562608 |
| Anti-mouse CD4 AF700 | BioLegend; clone RM4-5 | Cat# 56-0042-82, RRID:AB_494000 |
| Anti-mouse CD44 BV650 | BioLegend; clone IM7 | Cat# 103049, RRID:AB_2562600 |
| Anti-mouse CD44 APC-eF780 | Thermo Fisher; clone IM7 | Cat# 47-0441-80, RRID:AB_1272248 |
| Anti-mouse KLRG1 BV421 | BD; clone 2F1 | Cat# 566284, RRID:AB_273965 |
| Anti-mouse KLRG1 PerCP Cy 5.5 | BioLegend; clone 2F1 | Cat# 138418, RRID:AB_2563015 |
| Anti-mouse KLRG1 PE-Cy7 | BioLegend; clone 2F1 | 138416, RRID:AB_2561736 |
| Anti-mouse KLRG1 APCe780 | Thermo Fisher; clone 2F1 | Cat# 47-5893-82, RRID:AB_2573988 |
| Anti-mouse PD-1, PE-Cy7 | BioLegend; clone RMP1-30 | Cat# 109110, RRID:AB_572017 |
| Anti-mouse PD-1, BV786 | BD; clone RMP1-30 | Cat# 748264, RRID:AB_2872692 |
| Anti-mouse B220 Dy395XL [conjugated in house] | BioLegend; clone RA3-6B2 | Cat# 103202, RRID:AB_312987 |
| Anti-mouse B220 BV650 | BioLegend; clone RA3-6B2 | Cat# 103241, RRID:AB_11204069 |
| Anti-mouse B220 PerCP-Cy5.5 | BioLegend; clone RA3-6B2 | Cat# 103236, RRID:AB_89335 |
| Anti-mouse NOS2 AF405 | Santa Cruz Biotechnology; clone C-11 | Cat# sc-7271, RRID:AB_627810 |
| Anti-purified protein derivative | Abcam; polyclonal | ab905, RRID:AB_306965 |
| Anti-mouse CD90.1 eF450 | Thermo Fisher; clone HIS51 | Cat# 48-0900-82, RRID:AB_1272254 |
| Anti-mouse CD90.1 AF647 | BioLegend; clone OX-7 | Cat# 202507, RRID:AB_492885 |
| Anti-mouse pSMAD3 CF594 [conjugated in house] | Abcam; clone EP823Y | Cat# ab52903, RRID:AB_882596 |
| Anti-mouse Ki67 e506 | Thermo Fisher; clone SolA15 | Cat# 69-5698-82, RRID:AB_2637482 |
| Anti-mouse Ki67 PE-Cy7 | Thermo Fisher; clone SolA15 | Cat# 25-5698-82, RRID:AB_11220070 |
| Anti-mouse TNF AF700 | BioLegend; clone MP6-XT22 | Cat# 506338, RRID:AB_2562918 |
| Anti-mouse T-bet, AF488 | BioLegend; clone 4B10 | Cat# 644829, RRID:AB_2566018 |
| Anti-mouse T-bet, PE-Cy7 | BioLegend; clone 4B10 | Cat# 644823, RRID:AB_2561760 |
| Anti-mouse IL-2, PE | Thermo Fisher; clone JES6-5H4 | Cat# 12-7021-41, RRID:AB_11042257 |
| Anti-mouse IL-2, PE-Cy7 | Thermo Fisher; clone JES6-5H4 | Cat# 25-7021-82, RRID:AB_1235004 |
| Anti-mouse IRF4 CF594 [conjugated in house] | BioLegend; clone IRF4.3E4 | Cat# 646402, RRID:AB_2280462 |
| Anti-mouse RORγt AF488 | Thermo Fisher; clone B2D | Cat# 53-6981-82, RRID:AB_2811877 |
| Anti-mouse RORγt PE | Thermo Fisher; clone AFKJS-9 | Cat# 12-6988-80, RRID:AB_1257212 |
| Anti-mouse Jojo-1 iodide | Invitrogen | Discontinued |
| Anti-mouse Foxp3 e450 | Thermo Fisher; clone FJK-16s | Cat# 48-5773-82, RRID:AB_1518812 |
| Anti-mouse Foxp3 AF700 | Thermo Fisher; clone FJK-16s | Cat# 56-5773-80, RRID:AB_469950 |
| Anti-human Myeloperoxidase | Dako; clone A0398 | Cat# A0398, RRID:AB_2335676 |
| Anti-human CD68 | Biocare; clone KP1 | Cat# 033, RRID: RRID:AB_2885063. |
| Anti-human CD163 | Thermo Fisher; clone 10D6 | Cat# MA5-11458, RRID:AB_10982556 |
| Anti-human CD4 | Abcam; clone EPR6855 | Cat# ab195842, RRID:AB_2819211 |
| Bacterial and Virus Strains | ||
| M. tuberculosis H37Rv | Obtained from Joel Ernst (NYU) | ATCC 27294 |
| M. tuberculosis Erdman | BEI Resources | Cat# NR-15404 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ESAT-6 (4-17) peptide (QQWNFAGIEAAASA) | GeneMed Synthesis | N/A |
| OVA (323-339) peptide (ISQAVHAAHAEINEAGR) | GeneMed Synthesis | N/A |
| Fingolimod | Sigma-Aldrich | Cat# SML0700 |
| Mix-n-Stain CF Dye Antibody Labeling Kits | Biotium | Cat# 92433-92339 |
| Critical Commercial Assays | ||
| EasySep Mouse CD4+ T Cell Isolation Kit | StemCell Technologies | Cat# 19852 |
| Dead Cell Apoptosis Kit with Annexin V Pacific Blue and Sytox Green | Thermo Fisher | Cat# A35122 |
| Deposited Data | ||
| RNAseq of pulmonary ESAT-6-specific T cells | This paper | GEO GSE134186 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | JAX: 000664 |
| Mouse: ESAT-6 TCR transgenic (C7) | Obtained from Eric Pamer (MSK) | |
| Mouse: OVA TCR transgenic (OTII) | Obtained from Pamela Fink (UW) | |
| Rhesus macaque (Macaca mulatta) | Oregon National Primate Research Center | |
| Software and Algorithms | ||
| FlowJo | BD | https://www.flowjo.com/ |
| Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| CytoMAP | Stoltzfus et al., 2020 | https://gitlab.com/gernerlab/cytomap |
| LASX | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| Imaris | Bitplane | https://imaris.oxinst.com/ |
| Other | ||
| ESAT-64-17:I-Ab tetramer (mouse) PE [generated and tetramerized in house] | Construct from Marc Jenkins (University of Minnesota) | |
| ESAT-64-17:I-Ab tetramer (mouse) APC [generated and tetramerized in house] | Construct from Marc Jenkins (University of Minnesota) | |