Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in both PCRsa
| Primer | Sequence | Reference |
|---|---|---|
| CLA 18 | AGTCGGGACCTAAGGCGAG | 18 |
| CLA 21 | TTCCCGCTTAGATGCTTTCAG | 18 |
| CLA 3 | AGGTCCACGGGGTCTTG | This study |
Primers CLA 18 and CLA 21 were used in PCR-RFLP to obtain a 1.4-kbp amplified fragment. Primers CLA 18 and CLA 3 were used in 3′-mismatched PCR to obtain a 700-bp amplified fragment.
