Table 2.
Barcode Name | Primer Name | Sequence (5′-3′) | Annealing Temp | Amplicon (bp) |
---|---|---|---|---|
RbcL | rbcL-a-F | ATGTCACCACAAACAGAGACTAAAGC | 55 C | 560 |
rbcL-a-R | GTAAAATCAAGTCCACCRCG | |||
TrnL | trnL(UAA)-c | CGAAATCGGTAGACGCTACG | 50 C | 500 |
trnL(UAA)-d | GGGGATAGAGGGACTTGAAC | |||
psbA | psbA-trnH –F | GTTATGCATGAACGTAATGCTC | 64 C | 430 |
psbA-trnH-R | CGCGCATGGTGGATTCACAATCC | |||
MatK | matK-1RKIM-F | ACCCAGTCCATCTGGAAATCTTGGTTC | 52 C | 800 |
matK-3FKIM-R | CGTACAGTACTTTTGTGTTTACGAG | |||
ITS | ITS2-F | ATGCGATACTTGGTGTGAAT | 56 C | 460 |
ITS2-R | GACGCTTCTCCAGACTACAAT |