Figure 1.

Metabolic pathway analysis plot. Color intensity (white to red) reflects increasing statistical significance, and circle diameter varies with the pathway impact. The graph was made by plotting on the y-axis the −log₁₀ transforms of p-values from the pathway enrichment analysis and on the x-axis the pathway impact values derived from the pathway topology analysis. The top three most significant pathways (p-value < 0.05; pathway impact (pi) > 0) are depicted: arginine biosynthesis (p-value = 0.0022868, pi = 0.08641); pyrimidine metabolism (p-value = 0.0022904, pi = 0.09611); cysteine and methionine metabolism (p-value = 0.032033, pi = 0.04138). The metabolomic analysis was repeated three times using different biological samples giving the same results.