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. 2021 Oct 23;10(11):2559. doi: 10.3390/foods10112559

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) The cross fractured eggshell surface showing the cuticle pore plugs as visualized by a scanning electron microscopy (SEM) at 1000×. The comparison of the eggshell cuticle pore plug parameters (B) Pore width, (C) Pore plug thickness, (D) Pore length, and (E) Eggshell thickness in eggs that were obtained from different housing system. SEM images (n = 18/housing system) were used to quantify cuticle pore plug parameters using the ImageJ software. The data shown corresponds to eggs from mid-lay hens. The values with different superscript letters (Tukey multiple means comparison) are significantly different (ANOVA; p < 0.05). The values represent the mean ± standard deviation from six independent experiments (n = 18/housing system).