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Experimental design. Mice were intratracheally infected with 3–4 × 105 of clinical strain P. aeruginosa RP73 embedded in agar beads or empty beads and were sacrificed after seven days. Lungs, stool, and the final straight portion of the rectum (mucosae + feces) were aseptically excised and processed for metagenomic analysis. Read-outs of the disease progression were body weight changes, health status and CFUs in the lung.
