Skip to main content
. 2021 Nov 18;22(22):12431. doi: 10.3390/ijms222212431

Figure 2.

Figure 2

TCDD enriched Lactobacillus species possessing bile salt hydrolase (bsh). The presence of bsh gene sequences were assessed in metagenomic caecum samples from male C57BL/6 mice following oral gavage with sesame oil vehicle or 0.3, 3, or 30 µg/kg TCDD every 4 days for 28 days using three independent cohorts (n = 3). (A) The presence (green boxes) or absence of bsh sequences detected in any of the metagenomic samples (n = 3) are denoted within the respective treatment groups. Significant increases (*) or decreases (@) in normalized bsh abundances (adj. p < 0.1) are denoted. Also denoted is significantly increased species (#) determined by taxonomic analysis that corresponded with respective RefSeq species bsh annotations. Significance was determined by Maaslin2 R package. (B) Volcano plot displaying log2(fold-changes) in relative abundance of species between vehicle and 30 µg/kg TCDD treatment groups versus -log(adjusted p-values [adj. p]). Red dots denotes bsh sequences detected in 30 µg/kg TCDD treatment group. Significance was determined by the DeSeq2 R package comparing only vehicle and 30 µg/kg TCDD groups. Red dashed lines are reference to −log(0.05) value for the y-axis and −1 and 1 for the x-axis.