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The steps of the subtractive proteomics, and the results obtained from each step. (A) represent the whole proteome of Y. pestis with the total number of proteins, homologous and non-homologous. (B) shows the whole proteome of Y. pestis with the total number of proteins, paralogous and non- paralogous. (C) whole proteome of Y. pestis with the total number of proteins, essential and non-essential. (E) final vaccine targets while (D) represent the cellular location of different proteins.
