Figure 2.

Effect of DAPTMGY on intercellular ROS generation and on the contents of Total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase 1 (GPX1) in UVB-stimulated (40 mJ/cm²) HaCaT cells. Cells were cultured with DAPTMGY (20, 50, and 100 µM) for 2 h and then cultured for another 24 h after UVB irradiation. (a) Intercellular ROS production was measured in intact HaCaT cells using DCFH-DA, and photos were captured under fluorescence inverted microscopy. (b) The relative DCF fluorescence intensity analysis was conducted using Image J (fluorescence experiments were performed four times; n = 3 per group). (c–e) The levels of T-SOD, CAT, and GPX1 were measured using biochemical assay kits and ELISA kits. Results are shown as the mean ± SD (n = 3). ^## p < 0.01 and ^### p < 0.001 compared to the blank group (untreated cells); * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the control group.