FIG. 1.

DNA polymerase activity of human RAD30B protein. (A) Purified human RAD30B protein (370 ng) was analyzed by electrophoresis on an SDS–10% polyacrylamide gel and visualized by silver staining. Protein size markers (lane M) are indicated on the left. (B) Purified human RAD30B protein (19 ng) was analyzed by Western blotting using a mouse monoclonal antibody against the His₆ tag. Protein size markers (lane M) are indicated on the right. (C) DNA polymerase assays were performed without (lane 1) or with (lane 2) purified human RAD30B (19 ng), using the 40-mer template DNA, 5′-AAGGAAGGAAGGAAGGAACGAAGAACATACTTCTTCTTCC-3′, annealed with the 5′ ³²P-labeled primer, 5′-GGAAGAAGAAGTATGTT-3′. Quantitation of extended primers is shown at the bottom of the gel. DNA size markers in nucleotides are indicated on the right.