FIG. 5.

Nucleotide incorporation by human Polι opposite a template T. (A) DNA templates used for polymerase reactions. The annealed primers were not labeled with ³²P at their 5′ ends. (B and C) Using primed templates as indicated, DNA synthesis was performed with Polι (7.4 ng) in the presence of 5 μM dNTPs and 10 μCi of [α-³²P]dATP (A, lanes 1 and 5), [α-³²P]dCTP (C, lanes 2 and 6), [α-³²P]dTTP (T, lanes 3 and 7), or [α-³²P]dGTP (G, lanes 4 and 8) individually. Incorporation of ³²P was quantitated relative to the lowest-density band detectable. In lane 1 of panel C, [α-³²P]dAMP incorporation was the sum of the 16-mer and 17-mer bands. DNA size markers in nucleotides are indicated on the left.