FIG. 7.

Mismatch extensions by human Polι. (A) Various primers labeled at their 5′ ends with ³²P were annealed to the indicated template, generating 12 possible mismatches at the primer 3′ ends and at the underlined template positions. Mismatched substrates were incubated with (+) or without (−) human Polι (3.7 ng) under standard polymerase assay conditions. DNA size markers in nucleotides are indicated on the sides. (B) Three 5′ ³²P-labeled primers were annealed to the template as shown. Primer extensions by human Polι (1 ng) were then performed under polymerase assay conditions using either dATP (A), dCTP (C), dTTP (T), or dGTP (G) or all four dNTPs (N₄) as indicated. DNA size markers in nucleotides are indicated on the right.