FIG. 8.
Efficient nucleotide insertion by human Polι opposite a template AP site. A 32P-labeled 17-mer primer was annealed to the undamaged template FY2 or annealed right before a template AP site as shown. The AP site is located at the X position. Polymerase assays were performed with increasing amounts of human Polι using 50 fmol of undamaged templates (FY2) (lanes 1 to 4) and the AP site-containing template (template AP) (lanes 5 to 7). Quantitation of extended primers is shown at the bottom of the gel. DNA size markers in nucleotides are indicated on the right.