Table 1.

Primer sequences, optimal annealing temperature (Ta), and amplicon size of Sanger sequencing for verification of the mutations identified in this study.

Mutation	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Ta (°C)	Size (bp)
BSN R1087Q	TGCGGGAGGAAGAGGAGCTGCTT	GGAGCGGTGTAGCTCCTCCATCT	60	227
PCLO S1535L	CTTCTGGCTCTCAGTACTGC	CCTTCCAGCAAGGACCATAA	60	318
PCLO H5142R	CCCACTCTTATGTTTGCCTCTC	GACCTTGATTGGTGAAGCCTG	60	214