Figure 8.

Sequence analysis of HuPR-1 and its overexpression conferred heat tolerance to transgenic Arabidopsis plants. (A) Protein sequence alignment result of HuPR-1 in NCBI and protein alignment by Vector NTI Advance 11. (B) The qRT-PCR was used to measure the expression levels of HuPR-1 in transgenic Arabidopsis plants. (C) Survival rates (%) of WT and transgenic seedlings after heat treatment. Seven-day-old seedlings were heat-treated at 44 °C for 2 h and returned to 22 °C to grow for 2 days, and then photographed; following this, the survival rates were calculated. More than 50 plants of each line were analyzed. Bars represent standard deviations. Asterisks indicate statistically significant differences compared with wild type by Student’s t-test (* p < 0.05, ** p < 0.01).