Table 1.
Method validations for the five Alternaria mycotoxins in urine samples.
| Mycotoxins | LOD (ng/mL) |
LOQ (ng/mL) |
SSE (%) |
Spiked Levels (ng/mL) | Recovery (%) | Intra-Day Precision (%) | Inter-Day Precision (%) |
|---|---|---|---|---|---|---|---|
| AOH | 0.04 | 0.1 | 54.4 | 0.1 | 93 | 2.4 | 10.7 |
| 0.5 | 85 | 5.8 | 5.4 | ||||
| 5 | 91 | 2.1 | 5.2 | ||||
| AME | 0.02 | 0.05 | 70.0 | 0.05 | 75 | 6.7 | 8.0 |
| 0.2 | 93 | 3.4 | 2.3 | ||||
| 2 | 92 | 6.2 | 1.8 | ||||
| ALT | 0.2 | 0.5 | 88.7 | 0.5 | 104 | 5.9 | 10.8 |
| 2 | 101 | 3.5 | 4.3 | ||||
| 10 | 100 | 9.9 | 4.6 | ||||
| TeA | 0.05 | 0.1 | 58.7 | 0.1 | 77 | 7.6 | 11.8 |
| 0.5 | 90 | 6.7 | 7.7 | ||||
| 5 | 97 | 9.6 | 11.7 | ||||
| TEN | 0.02 | 0.05 | 84.1 | 0.05 | 90 | 9.5 | 8.6 |
| 0.2 | 90 | 2.6 | 3.3 | ||||
| 2 | 101 | 2.3 | 3.3 |
AOH = alternariol; AME = alternariol monomethyl ether; ALT = altenuene; TeA = tenuazonic acid; TEN = tentoxin; LOD = limit of detection, determined at signal-to-noise ratio (S/N) = 3; LOQ = limit of quantification, determined at S/N = 10; SSE = signal suppression/enhancement, calculated by comparing the slope of the matrix-matched calibration curve with that of the standard calibration curve; recovery, intra- and inter-day precision, performed in five replicates using blank urine samples spiked with low, intermediate and high concentration levels.