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. 2021 Nov 22;22(22):12559. doi: 10.3390/ijms222212559

Figure 3.

Figure 3

Effect of 100 µM ML218 HCl and 150 µM capsaicin [3] on Cal 27 cell metabolism. Cal 27 cells were either with (a) no antigen pre-stimulation, (b) 24 h or (c) 72 h bacterial antigens pre-stimulation prior to the addition of inhibitory drugs with and without additional antigen. M = ML218 HCL, C = capsaicin, LPS = lipopolysaccharide, LL = LPS + LTA (lipoteichoic acid). Letter before the arrow represents pre-stimulation, e.g., LPS -> M is LPS pre-stimulation and then treatment with ML218 HCL (M). n = nine biological replicates. Error bars represent standard error of the mean. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.