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. 2021 Nov 22;22(22):12559. doi: 10.3390/ijms222212559

Figure 6.

Figure 6

TNFα ELISA. Comparison of TNFα (pg/mL) under different treatment conditions. After 72 h stimulation of Cal 27 cells with LPS + LTA, Cal 27 cells were treated with drugs for 24 h in the absence or presence of bacterial antigens. M: ML218 HCl, C: capsaicin [3], LL = LPS + LTA. Letter before the arrow represents pre-stimulation, e.g., LL-> M is LL pre-stimulation and then treatment with ML218 HCl. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001 is statistically significant. n = nine biological replicates. Error bars represent standard error of the mean. All of the columns look rather the same although they are mathematically different.