Table 3.
Sensitivity of the DAS-ELISA test in different citrus species and apple using the antiserum against NP protein of CCGaV.
| Dilution a | Lemon | Clementine | Sweet Orange | Apple | |||||
|---|---|---|---|---|---|---|---|---|---|
| OD (SD) b | I/NI c | OD (SD) b | I/NI c | OD (SD) b | I/NI c | OD (SD) b | I/NI c | ||
| 1/10 | 1 mg | 1.653 (0.114) | 551 | 1.196 (0.068) | 149 | 0.818 (0.022) | 818 | 0.301 (0.030) | 301 |
| 1/20 | 500 µg | 1.306 (0.008) | 435 | 0.894 (0.080) | 112 | 0.610 (0.006) | 610 | 0.168 (0.012) | 168 |
| 1/40 | 250 µg | 0.934 (0.124) | 311 | 0.523 (0.089) | 65 | 0.326 (0.005) | 326 | 0.081 (0.005) | 81 |
| 1/80 | 125 µg | 0.673 (0.144) | 224 | 0.328 (0.098) | 41 | 0.184 (0.006) | 184 | 0.042 (0.003) | 42 |
| 1/160 | 62.5 µg | 0.365 (0.028) | 122 | 0.143 (0.027) | 18 | 0.110 (0.014) | 110 | 0.019 (0.003) | 19 |
| 1/320 | 31.3 µg | 0.195 (0.023) | 65 | 0.083 (0.030) | 10 | 0.054 (0.006) | 54 | 0.010 (0.003) | 10 |
| 1/640 | 15.1 µg | 0.100 (0.012) | 33 | 0.040 (0.009) | 5 | 0.0023 (0.001) | 23 | 0.005 (0.004) | 5 |
| Negative control (NI) | 0.003 (0.003) | 0.008 (0.012) | 0.001 (0.001) | 0.001 (0.005) | |||||
a Crude extracts from infected tissues were diluted in two-fold steps from 1/10 to 1/640 using noninfected (NI) leaf extracts as diluent. The corresponding amounts of infected tissues for each dilution are reported on the right column. b Mean Optical Density (OD) at 405 nm for ten technical replicates measured after 30 min of incubation. In brackets is the standard deviation (SD). c Ratio between OD in CCGaV-infected (I) and OD in the control noninfected plant (NI).