Table 4.
Sensitivity of DAS-ELISA test in different apple tissues using the antiserum against NP protein of CCGaV.
| Dilution a | Petals | Young Leaves | Mature Leaves | Buds | |||||
|---|---|---|---|---|---|---|---|---|---|
| OD (SD) b | I/NI c | OD (SD) b | I/NI c | OD (SD) b | I/NI c | OD (SD) b | I/NI c | ||
| 1/10 | 1 mg | 1.076 (0.011) | 1076 | 0.886 (0.132) | 886 | 1.242 (0.092) | 1242 | 1.824 (0.049) | 1824 |
| 1/20 | 500 µg | 0.685 (0.052) | 685 | 0.461 (0.016) | 461 | 0.752 (0.021) | 752 | 1.249 (0.075) | 1249 |
| 1/40 | 250 µg | 0.301 (0.013) | 301 | 0.238 (0.006) | 238 | 0.366 (0.012) | 366 | 0.665 (0.024) | 665 |
| 1/80 | 125 µg | 0.161 (0.027) | 161 | 0.139 (0.004) | 139 | 0.224 (0.011) | 224 | 0.397 (0.037) | 307 |
| 1/160 | 62.5 µg | 0.081 (0.012) | 81 | 0.060 (0.012) | 60 | 0.106 (0.010) | 106 | 0.182 (0.047) | 182 |
| 1/320 | 31.3 µg | 0.036 (0.001) | 36 | 0.039 (0.006) | 39 | 0.060 (0.013) | 60 | 0.076 (0.009) | 76 |
| 1/640 | 15.1 µg | 0.017 (0.005) | 17 | 0.019 (0.004) | 19 | 0.025 (0.004) | 25 | 0.036 (0.003) | 36 |
| Negative control (NI) | 0.001 (0.002) | ||||||||
a Crude extracts from infected tissues were diluted in two-fold steps from 1/10 to 1/640 using noninfected (NI) leaf extracts as diluent. The corresponding amounts of infected tissues for each dilution are reported in the right column. b Mean Optical Density (OD) at 405 nm for ten technical replicates measured after 30 min of incubation. In brackets is the standard deviation (SD). c Ratio between OD in CCGaV-infected (I) and OD in the control noninfected plant (NI).