Figure 1.

HPLC and CE profiles present in the eight subjects with Hb A₂ variants: case numbering corresponds to that shown in Table 1. Case 6 shows the profile in which the Hb A₂-Asti or (α₂δ₂^Asti) is not visible as co-migrating with Hb A. Instead, we can observe Hb A₂^J-Rovigo or (α₂^J-Rovigo δ₂) is not quantifiable, but visible as a basic movement in the “Hb F zone” and produced by the presence of α^J-Rovigo chains that, with the normal delta chains, form a mutated tetramer. No Hb A₂ variant can be observed for cases 1 and 7.