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Isothermal titration calorimetry analysis of Tween® 20 and β-glucosidase. (A) Raw titration data of Tween® 20 (0.5 mM) titrated over β-Glu (37 µM) in PBS buffer, pH 6. (B) Binding isotherm of Tween® 20 titration over β-Glu, obtained by integrating raw data for the protein titration. The blue dots represent the experimental data and the red curve represents the fit of the data using a single set of sites model.
