Table 4.

Physical characterization and loading content of Tween^® 20:β-Glucosidase solutions in PLGA NPs.

	Z-Average nm ± S.D.	PDI ± S.D.	AFM Diameter nm ± S.D.	ζ-Pot mV ± S.D.	Yield% ± S.D.	LC% ± S.D.	EE% ± S.D.
Empty NPs	190 ± 15	0.06 ± 0.01	320 ± 47	−20 ± 3	85.1 ± 3.1	-	-
NPs_Solution0	199 ± 25	0.09 ± 0.02	311 ± 69	−22 ± 6	82.5 ± 6.8	0.4 ± 0.1	3.9 ± 1.5
NPs_Solution1	185 ± 24	0.08 ± 0.03	173 ± 41	−16 ± 5	72.7 ± 5.5	1.1 ± 0.0	9.7 ± 1.2
NPs_Solution2	188 ± 13	0.08 ± 0.01	142 ± 39	−16 ± 10	73.9 ± 4.3	1.0 ± 0.1	11.1 ± 1.2
NPs_Solution3	191 ± 23	0.08 ± 0.02	150 ± 26	−16 ± 7	81.8 ± 3.6	0.8 ± 0.1	8.4 ± 4.0
NPs_Solution4	198 ± 33	0.09 ± 0.06	270 ± 31	−16 ± 7	75.9 ± 2.3	0.9 ± 0.1	7.8 ± 2.5