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. 2021 Nov 2;9(11):289. doi: 10.3390/toxics9110289

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Percentage (%) of DNA damage (mean ± SD) in tail in liver and gills of Danio rerio (n = 3 pools of 10 individuals) and Perca fluviatilis (n = 6). Six slides per pool (zebrafish) and six slides per individual (perch) were measured, in order to represent technical replicates. Randomly selected 100 cells were scored from each slide (TritekCometscoreTM 1.5, TriTek Corporation, Wilmington, DE, USA). Representative pictures of DNA damage are shown. Mann–Whitney U test was employed to test for significance at p < 0.05 between all experimental groups. * denotes significant differences (p < 0.05) compared to the control group (n = 3 pools of 10 individuals and n = 6 for D. rerio and P. fluviatilis respectively). % DNA in tail and Olive moment in positive control data (1 μΜ H₂O₂) were 28.3 ± 5.2 and 40 ± 6.3, respectively.