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. 2021 Nov 22;14(11):1196. doi: 10.3390/ph14111196

Figure 5.

Figure 5

mRNA expression in human adipocytes (HASCs) treated with MLR-1023 and cold stimulation. qRT-PCR assays were conducted in duplicates following MLR-1023 (60 µM) treatment of human adipocytes from three obese donors (four technical replicates each) for seven days compared to untreated adipocytes (control), maintained at 37 °C, or treated as such and maintained at 26 °C for the last 24 h: (A) uncoupling protein 1 (UCP1) and (B) protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2β). The results are presented as mean ± SE. * p < 0.05, *** p ≤ 0.001 compared to the respective control.