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. 2021 Nov 22;14(11):1196. doi: 10.3390/ph14111196

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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mRNA expression in human adipocytes (HASCs) treated with MLR-1023 and menthol. qRT-PCR assays conducted in duplicates following MLR-1023 (30 µM), menthol (30 µM), and MLR-1023 in addition to menthol (30 µM each) treatment of human adipocytes from three obese donors (four technical replicates each) compared to untreated adipocytes (control): (A) uncoupling protein 1 (UCP1) and (B) glucose transporter type 4 (GLUT4). The results are presented as mean ± SE. * p < 0.05, *** p < 0.001 compared to the respective control. MLR-1023 and menthol also exhibit synergy, where the combined effect compared to control is greater than the sum of the effects of MLR-1023 or menthol alone, compared to the control.