Figure 2.

Synthesis and characterization of sEV-derived sEV-like vesicles (ELVs) using a modified thin-film-hydration (TFH) method. (A) Workflow of ELV synthesis from CPC-sEVs by depleting sEV cargo, forming a lipid film and rehydrating with customized microRNA (miRNA). (B) Transmission-electron microscopy image of an engineered ELV. Scale bar = 100 nm. (C) Concentration-size profile of an ELV with NTA. n = 10. (D) Comparison of ELV and sEV size and concentration. (E) Comparison of percentage poly-dispersity of ELVs and sEVs. n = 4–8 represent biological replicates. Mean ± SEM. Significance was tested with two-way, Student’s paired t-test. n.s. = not significant.