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. 2021 Oct 22;8(11):135. doi: 10.3390/jcdd8110135

Figure 5.

Figure 5

miR-126+ ELVs induce pro-angiogenic response in CECs. (A) Schematic of miR-126 mechanism of action for angiogenesis. VEGF: vascular endothelial growth factor; RAF1: rapidly accelerated fibrosarcoma proto-oncogene; ERK: extracellular signal-regulated kinase; AKT: protein kinase B; PI3K: Phosphoinositide 3-Kinase. (B) CECs (green) treated with miR-126+ ELVs or sEVs incubated on Matrigel form tubes after overnight incubation. CECs treated with calcein-AM. Scale bar = 200 µm. (C) Quantification of angiogenic parameters (ImageJ software version 2.1.0/1.53 g) shows increase in number of tubules, total tube length and segment length for CECs treated with miR-126+ ELVs compared to sEVs. Data normalized to negative control. n = 6–7 represent biological replicates. Mean ± SEM. Significance was tested with two-way Student’s paired t-test. * p ≤ 0.05, ** p ≤ 0.01.