Table 3.
(A) Composition of tryptic soy broth (and tryptic soy broth supplemented with glucose) according to manufacturer’s specification. 10 *—concentration of glucose monohydrate in tryptic soy broth supplemented with glucose (g/L). (B) Composition of DMEM.
| (A) Tryptic Soy Broth (TSB) | g/L |
| Proteins | |
| Pancreatic digest of casein | 17 |
| Peptic digest of soybean | 3 |
| Inorganic salts | |
| Dipotassium hydrogen phosphate | 2.5 |
| Sodium chloride | 5 |
| Other components | |
| Glucose monohydrate | 2.5/10 * |
| (B) Dulbecco’s Modified Eagle’s Medium (DMEM) High Glucose L0103-500 Liquid | g/L |
| Amino acids | |
| Glycine | 0.03 |
| L-Alanyl-L-glutamine (glutamine stable) | 0.862 |
| L-Arginine monohydrochloride | 0.084 |
| L-Cystine dihydrochloride | 0.0626 |
| L-Histidine monohydrochloride monohydrate | 0.042 |
| L-Isoleucine | 0.105 |
| L-Leucine | 0.105 |
| L-Lysine monohydrochloride | 0.146 |
| L-Methionine | 0.03 |
| L-Phenylalanine | 0.066 |
| L-Serine | 0.042 |
| L-Threonine | 0.095 |
| L-Tryptophan | 0.016 |
| L-Tyrosine disodium salt dihydrate | 0.10379 |
| L-Valine | 0.094 |
| Inorganic salts | |
| Calcium chloride dihydrate | 0.265 |
| Ferric nitrate nonahydrate | 0.0001 |
| Magnesium sulfate anhydrous | 0.09767 |
| Potassium chloride | 0.4 |
| Sodium bicarbonate | 3.7 |
| Sodium chloride | 6.4 |
| Sodium phosphate monobasic anhydrous | 0.109 |
| Vitamins | |
| Choline chloride | 0.004 |
| D-Ca pantothenate | 0.004 |
| Folic acid | 0.004 |
| Myo-inositol | 0.0072 |
| Nicotinamide | 0.004 |
| Pyridoxal hydrochloride | 0.004 |
| Riboflavine | 0.0004 |
| Thiamine hydrochloride | 0.004 |
| Other components | |
| D-Glucose anhydrous | 4.5 |
| Phenol red solution salt | 0.0159 |
| Sodium pyruvate | 0.11 |