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. 2021 Nov 8;10:e70843. doi: 10.7554/eLife.70843

Table 2. Multiple reaction monitoring (MRM) transitions and mass spectrometry (MS) method details target nucleocapsid protein (NCAP) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) peptides.

Peptide MRM MRM transition type Cone voltage (V) Collision energy (V) Retention time (min) Scan window (min)
ADETQALPQR 564.8 > 400.2 Quantifier 35 19 1.09 0.6–1.4
564.8 > 584.4 Qualifier 35 20
564.8 > 712.4 Qualifier 35 24
569.8 > 410.2 SIL 35 19
AYNVTQAFGR 563.8 > 679.4 Quantifier 35 19 2.49 2.0–3.0
563.8 > 578.3 Qualifier 35 18
563.8 > 892.5 Qualifier 35 19
568.8 > 689.4 SIL 35 19
DGIIWVATEGALNTPK 562.3 > 643.4 Quantifier 35 14 4.12 3.6–4.8
562.3 > 572.3 Qualifier 35 18
562.3 > 700.4 Qualifier 35 14
565.2 > 708.4 SIL 35 14
NPANNAAIVLQLPQGTTLPK 687.4 > 841.5 Quantifier 35 18 3.92 3.6–4.2
687.4 > 766.4 Qualifier 35 23
687.4 > 865.5 Qualifier 35 23
690.4 > 849.5 SIL 35 18