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. 2021 Nov 8;10:e67952. doi: 10.7554/eLife.67952

Figure 5. H3K4 methylation by the extended catalytic module (eCM) of the complex of proteins associated with Set1 (COMPASS) methyltransferase complex is inhibited by H4 sumoylation.

(A) Structure of the COMPASS eCM bound to a mononucleosome (PDB code 6UGM). The disordered H3 and H4 tails are shown in gold and blue, respectively, with the last observable amino acid indicated. Dotted lines indicate missing N-terminal amino acids. Spp1 (orange) suppressor of PRP protein 1. Swd3 (light blue), Set1 complex WD40 repeat protein 3. Swd1 (red), Set1 complex WD40 repeat protein 1. Set1 (green), SET domain protein 1. Bre2 (pink), brefeldin-A sensitivity protein 2. Sdc1-A/B (yellow), suppressor of CDC25 protein 1. (B) Western blots of the products from methylation assays with mononucleosome substrates containing wild-type (wt) H4 or H4K12su and the COMPASS eCM complex. Mono-, di-, and trimethylated states of H3K4 were detected by the indicated modification-specific antibodies. (C) Western blots of the products from methylation assays with mononucleosome substrates containing wt H4 or H4K12su and the COMPASS catalytic module. Mono-, di-, and trimethylated states of H3K4 were detected by the indicated modification-specific antibodies.

Figure 5—source data 1. Unedited western blot membranes for all western blot images shown in Figure 5.

Figure 5.

Figure 5—figure supplement 1. Coomassie-stained SDS-PAGE gel of recombinant Kluyveromyces lactis complex of proteins associated with Set1 (COMPASS) catalytic module (CM) and COMPASS extended catalytic module (eCM) sub-complexes used in H3K4 methylation assays.

Figure 5—figure supplement 1.

The Sdc1 subunit (10 kDa) is not observed on this gel due to its size. Set1-SET is the catalytic domain. Set1-N674 includes the nSET domain, beginning at residue 674.