. 2021 Nov 8;10:e67952. doi: 10.7554/eLife.67952

Table 3. Comparisons of relative ion intensities of characteristic fragment ions from an enzymatically tri-acetylated and chemically propionylated H4(4–17) peptide, [M + 2 H]²⁺ = 755 Da, after activator and p300-mediated acetylation of chromatinized plasmids containing wild-type (wt) H4^*_.

Species	Ion	% of Total ion intensity			Avg. ratio
K5ac, K8ac, K12ac	b₉⁺	3.032	4.938	3.981	24.1 ± 13.3
K5ac, K8ac, K12pr	b₉⁺	0.339	0.096	0.147	24.1 ± 13.3
K5ac, K8ac, K12ac	b₁₀⁺	0.875	0.862	1.109	10.3 ± 5.1
K5ac, K8ac, K12pr	b₁₀⁺	0.112	0.152	0.064	10.3 ± 5.1
K12pr, K16ac	y₈⁺	0.271	n.d.	0.012	0.2
K12ac, K16pr	y₈⁺	0.885	1.005	1.263	0.2
K12pr, K16ac	y₉⁺	n.d.	0.123	0.054	0.02
K12ac, K16pr	y₉⁺	2.587	4.442	6.296	0.02

Three unique spectra corresponding to the tri-acetylated and propionylated H4(4–17) tail peptide from wild-type (wt) H4 chromatin were analyzed. Error reported is standard deviation of the mean. n.d. = not detected.

Table 3. Comparisons of relative ion intensities of characteristic fragment ions from an enzymatically tri-acetylated and chemically propionylated H4(4–17) peptide, [M + 2 H]2+ = 755 Da, after activator and p300-mediated acetylation of chromatinized plasmids containing wild-type (wt) H4*.

Table 3. Comparisons of relative ion intensities of characteristic fragment ions from an enzymatically tri-acetylated and chemically propionylated H4(4–17) peptide, [M + 2 H]²⁺ = 755 Da, after activator and p300-mediated acetylation of chromatinized plasmids containing wild-type (wt) H4^*_.