Extended Data Figure 3. Endothelial Pten LOF synergizes with Krit1 LOF in a dose-dependent manner.

a, Schematic of neonatal endothelial induction with tamoxifen of Krit1 deletion and deletion of either none or one allele of Pten in endothelial cells using a Pdgfb-CreERT2 transgene. b, Representative visual and paired microCT images of Krit1^iECKO and Krit1^iECKO;Pten^fl/+ littermate mice on a susceptible microbiome background at P12. Scale bars, 1mm. These mice were produced from a Pten^fl/+ x Pten^fl/+ cross; however no Krit1^iECKO;Pten^fl/fl littermates survived to P12. c, MicroCT quantification of lesion volumes at P12. (Krit1^iECKO, n=6; Krit1^iECKO;Pten^fl/+, n=13). d, Schematic of neonatal endothelial induction of Krit1 deletion and deletion of either one or both alleles of Pten in endothelial cells using a Pdgfb-CreERT2 transgene with brains harvested at P7. These mice were produced from a Pten^fl/+ x Pten^fl/fl cross. e, Representative visual and paired microCT images in Krit1^iECKO;Pten^fl/+ and Krit1^iECKO;Pten^iECKO littermate mice at P7. Scale bars, 1mm. f, MicroCT quantification of lesion volumes at P7. (Krit1^iECKO;Pten^fl/+, n=13; Krit1^iECKO;Pten^iECKO, n=8). Data are mean ± s.e.m. Unpaired, two-tailed Welch’s t-test.