REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
Ammonium bicarbonate | Sigma-Aldrich | A6141 |
Sodium dodecyl sulfate | Sigma-Aldrich | L3771 |
Dithiothreitol | Sigma-Aldrich | D0632 |
Iodoacetamide | Acros Organics | 122270050 |
Trypsin protease, MS grade | Thermo Fisher Scientific | 90305 |
Trifluoroacetic acid | Sigma-Aldrich | T6508 |
Acetonitrile | JT Baker | 14650359 |
Formic acid | Sigma-Aldrich | F0507 |
Urea | Sigma-Aldrich | U0631 |
LC-MS grade water | Sigma-Aldrich | 39253 |
Bovine serum albumin | Thermo Fisher Scientific | 23208 |
Acetone | JT Baker | 9006–03 |
Acrylamide | Thermo Fisher Scientific | AM9022 |
SDS | Thermo Fisher Scientific | 15525017 |
APS | Thermo Fisher Scientific | HC2005 |
TEMED | Thermo Fisher Scientific | T9281 |
Tris-Base | Thermo Fisher Scientific | 17926 |
HCL | Thermo Fisher Scientific | 24308 |
Glycine | Thermo Fisher Scientific | A37730IN |
Glacial acetic acid | Sigma-Aldrich | A0808 |
Coomassie Brilliant Blue | Thermo Fisher Scientific | 20278 |
ß-Mercaptoethanol | Sigma-Aldrich | M6250 |
Bradford reagent | Thermo Fisher Scientific | 22663 |
Software and algorithms | ||
Proteome Discoverer 2.2 using Sequest HT | Thermo Fisher Scientific | OPTON-30945 |
Xcalibur | Thermo Fisher Scientific | OPTON-30965 |
Uniprot | (https://www.uniprot.org): | |
Others | ||
Benchtop centrifuge | Eppendorf | 5427R |
Sonicator | Helix Biosciences | HBSNII-92 |
Vortex | Sigma-Aldrich | Z258423 |
Incubator | Memmert | INB200 |
Vacuum evaporator | Genevac | DNA-12060-C00 |
Microplate reader | Thermo Fisher Scientific | VL0000D0 |
Nano Reverse-phased capillary HPLC system | Thermo Fisher Scientific | UltiMate 3000 HPLC |
Q-exactive + orbitrap | Thermo Fisher Scientific | IQLAAEGAAPFADBMBCX |
Heated electrospray ionization (H-ESI) ion source | Thermo Fisher Scientific | H-ESI probe |
SDS PAGE Apparatus | Bio-Rad Laboratories | 165-8000 |
Viral Transfer media | RMBio | VTM-CHT |
Eppendorf tubes | Eppendorf | 30125150 |
ELISA plates | Thermo Fisher Scientific | 44-2404-21 |
Pierce C18 spin columns | Thermo Fisher Scientific | 89870 |
Water bath | LabcOM | 76308-830 |
Nano LC Column | Thermo Fisher Scientific | 164570 |
Calibration solution | Thermo Fisher Scientific | 88323/88324 |