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. 2021 Nov 27;3(1):101045. doi: 10.1016/j.xpro.2021.101045
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Ammonium bicarbonate Sigma-Aldrich A6141
Sodium dodecyl sulfate Sigma-Aldrich L3771
Dithiothreitol Sigma-Aldrich D0632
Iodoacetamide Acros Organics 122270050
Trypsin protease, MS grade Thermo Fisher Scientific 90305
Trifluoroacetic acid Sigma-Aldrich T6508
Acetonitrile JT Baker 14650359
Formic acid Sigma-Aldrich F0507
Urea Sigma-Aldrich U0631
LC-MS grade water Sigma-Aldrich 39253
Bovine serum albumin Thermo Fisher Scientific 23208
Acetone JT Baker 9006–03
Acrylamide Thermo Fisher Scientific AM9022
SDS Thermo Fisher Scientific 15525017
APS Thermo Fisher Scientific HC2005
TEMED Thermo Fisher Scientific T9281
Tris-Base Thermo Fisher Scientific 17926
HCL Thermo Fisher Scientific 24308
Glycine Thermo Fisher Scientific A37730IN
Glacial acetic acid Sigma-Aldrich A0808
Coomassie Brilliant Blue Thermo Fisher Scientific 20278
ß-Mercaptoethanol Sigma-Aldrich M6250
Bradford reagent Thermo Fisher Scientific 22663

Software and algorithms

Proteome Discoverer 2.2 using Sequest HT Thermo Fisher Scientific OPTON-30945
Xcalibur Thermo Fisher Scientific OPTON-30965
Uniprot (https://www.uniprot.org):

Others

Benchtop centrifuge Eppendorf 5427R
Sonicator Helix Biosciences HBSNII-92
Vortex Sigma-Aldrich Z258423
Incubator Memmert INB200
Vacuum evaporator Genevac DNA-12060-C00
Microplate reader Thermo Fisher Scientific VL0000D0
Nano Reverse-phased capillary HPLC system Thermo Fisher Scientific UltiMate 3000 HPLC
Q-exactive + orbitrap Thermo Fisher Scientific IQLAAEGAAPFADBMBCX
Heated electrospray ionization (H-ESI) ion source Thermo Fisher Scientific H-ESI probe
SDS PAGE Apparatus Bio-Rad Laboratories 165-8000
Viral Transfer media RMBio VTM-CHT
Eppendorf tubes Eppendorf 30125150
ELISA plates Thermo Fisher Scientific 44-2404-21
Pierce C18 spin columns Thermo Fisher Scientific 89870
Water bath LabcOM 76308-830
Nano LC Column Thermo Fisher Scientific 164570
Calibration solution Thermo Fisher Scientific 88323/88324