Tris's buffer (pH-6.8)

Reagent	Final concentration	Volume
Tris's base	0.5 M Tris's base in deionized water.	100 mL

Dissolve 6 gm of Tris base in 60 mL of deionized water, now adjust the pH to 6.8 using 6 N HCL and then adjust final volume to 100 mL.