Tris-Glycine buffer (Running buffer)

Reagent	Final concentration	Volume
Tris's base	250 mM
Glycine	1.92 M
SDS	1%
water	to 1 L	1 L

Dissolve 30.30 gm tris, 144.10 gm Glycine, and SDS 10 gm in 900 mL of water and make final volume 1 L.