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Staining solution
| Reagent | Final concentration | Volume |
|---|---|---|
| Coomassie blue R250 | 0.1% (w/v), | |
| methanol | 20% (v/v) | |
| Acetic acid | 10% (v/v) |
Dissolve 0.4 gm Coomassie blue in 200 mL of 40% (v/v) HPLC grade methanol in water with stirring as required. Filter the solution to remove any insoluble material. Add 200 mL of 20% (v/v) acetic acid in water.
