| Vial | Volume of diluent |
Volume and source vial of BSA |
Final concentration |
|---|---|---|---|
| (μL) | (μL) | (μg/μL) | |
| A | 0 | 300 of stock | 2000 |
| B | 125 | 375 of stock | 1500 |
| C | 325 | 325 of stock | 1000 |
| D | 175 | 175 of vial B dilution | 750 |
| E | 325 | 325 of vial C dilution | 500 |
| F | 325 | 325 of vial E dilution | 250 |
| G | 325 | 325 of vial F dilution | 125 |
| H | 400 | 100 of vial G dilution | 25 |
| I | 400 | 0 | 0 |
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