Fig. 2.

DSE and DLE do not affect the membrane integrity of MCF-7 cells (A) Changes in the membrane potential of MCF-7 cells were measured using a membrane potential-sensitive dye. Cells were previously incubated with the dye DiSC₃(5) (200 μM) for 30 min at 37 °C and then treated with DSE and DLE IC₅₀. Valinomycin (444.5 µg/mL) was used as a positive control. (B) Relative fluorescence intensities for intracellular calcium release. Measurements were performed for 5 min. PMA (1.8 µg/mL) was used as a positive control. Arrows indicate the time at which the treatments were added.