FIG. 8.

W37A mutation of Stat1 causes loss of IFN-α-induced DNA binding. U3A cells were infected with wild-type Stat1 (wt) or Stat1 with a W37A mutation. Retrovirally infected cells were purified by cell sorting and treated with IFN-α for 20 min. Nuclear extracts were prepared, and EMSA was performed with the indicated probes. Arrowheads are positioned to indicate the relative migration positions between gels: solid arrowheads indicate lower mobility and open arrowheads indicate higher mobility.