Table 2.
Relative abundances and calculated slopes of uncorrected and corrected acetylation timecourse data of the fully acetylated histone 3 peptide H3(18-26) resulting in 3 different isotopologues with either none (M + 0), one (M + 2) or two (M + 4) acetyl groups labelled
| uncorrected | PICor | AccuCor | ||
|---|---|---|---|---|
| t = 0 h | M + 0 | 84.1 ± 1.1% | 100.0 ± 0.0% | 97.0 ± 1.4% |
| M + 2 | 15.9 ± 1.1% | 0.0 ± 0.0% | 3.0 ± 1.4% | |
| M + 4 | 0.0 ± 0.0% | 0.0 ± 0.0% | 0.0 ± 0.0% | |
| t = 24 h | M + 0 | 28.3 ± 3.2% | 33.9 ± 4.0% | 32.6 ± 3.7% |
| M + 2 | 46.2 ± 0.8% | 46.7 ± 1.5% | 46.7 ± 1.3% | |
| M + 4 | 25.6 ± 2.5% | 19.4 ± 2.5% | 20.7 ± 2.5% | |
| Slope | M + 0 | − 2.33 ± 0.08%h− 1 | − 2.75 ± 0.10%h− 1 | − 2.69 ± 0.09%h− 1 |
| M + 2 | 1.26 ± 0.03%h− 1 | 1.94 ± 0.04%h− 1 | 1.82 ± 0.05%h− 1 | |
| M + 4 | 1.06 ± 0.06%h− 1 | 0.81 ± 0.06%h− 1 | 0.86 ± 0.06%h− 1 |
The values for the individual data points are listed with standard deviations and the estimated slopes are provided with standard error of the mean (n = 3)