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. 2021 Nov 26;12:6942. doi: 10.1038/s41467-021-27284-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — E. coli BL21(DE3) cells transformed with either an empty plasmid (blank) or a plasmid encoding AlkD, YtkR2, or C10R5 were grown in the presence of various concentrations of YTM, DSA, or CC-1065. A shorter lag phase (lag time) before the onset of exponential growth indicates increased drug resistance. A complete lack of cell growth was plotted as a lag time of 18 h, the total incubation period for the cultures, as indicated by the dashed line. Data are presented as the mean ± SD from four replicate experiments. Source data are provided as a Source Data file.