Fig. 7. Circulating and tissue-resident memory T cell subsets in the lung.

C57BL/6 mice were vaccinated according to Fig. 5A. Antigen-experienced CD8⁺ T cells were identified by CD44 staining and intravascular staining was used to differentiate between circulating (iv-labelled) and tissue-resident (iv-protected) memory cells. Representative contour plots are shown in (A). B The total number of CD44⁺ CD8⁺ with the relative contribution of iv− and iv+ cells are summarized for each group. C Within the iv-labelled CD44⁺ CD8⁺ population, effector T cells (T_EFF; CD127^-KLRG1⁺), effector memory T cells (T_EM; CD127⁺KLRG1⁺), and central memory T cells (T_CM; CD127⁺KLRG1⁻CD69⁻CD103⁻) were defined. Within the iv-protected population, T_RM cells were defined as KLRG1⁻CD103⁺CD69⁺. The gating strategy is shown in Supplementary Fig. 2. Bars represent group means overlaid with individual data points; all groups n = 4. Data were analysed by one-way ANOVA followed by Tukey’s multiple comparison test. Statistically significant differences are indicated only among the different vaccine groups; p values indicate significant differences (*p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001). Representative data from one out of three independent experiments with slightly different end time points are shown.