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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Arthritis Rheumatol. 2021 Oct 22;73(12):2200–2205. doi: 10.1002/art.41807

Figure 1. T cell responses to P. copri HLA-DR-presented peptides in RA patients.

Figure 1.

Five peptides derived from 5 P. copri (Pc) proteins were synthesized and used to stimulate PBMC from RA patients by IFN-γ ELISpot assays. Using TEPITOPE, the Pc-p27, Pc-ribonuclease HII, and Pc-DNAbinding peptides were predicted to be promiscuous binders of ≥20 of the 25 HLA-DR molecules modeled in the program. The predicted binding of the Pc-glutamate-5-kinase and Pc-type III restriction endonuclease peptides was restricted primarily to DRB1*04 molecules. The superscript numbers around each peptide sequence show the location of the amino acids within the source protein. The horizontal bar represents the mean value, and the grey area shows >3SD above the mean value in healthy control subjects (hospital personnel). The groups were compared using unpaired t test with Welch correction. SFU = spot forming units per 1 × 106 cells. RA = rheumatoid arthritis, and HC = healthy controls.